自动化高通量细胞微手术芯片。

Automated high-throughput cell microsurgery on-chip.

机构信息

School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

出版信息

Lab Chip. 2009 Oct 7;9(19):2764-6. doi: 10.1039/b910703g. Epub 2009 Aug 19.

PMID:19967110

Abstract

Laser cell kill is an established tool for studying cells' roles during development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of Caenorhabditis elegans and software for image processing and automation, allowing for high-throughput cell ablations.

摘要

激光杀死细胞是研究细胞在发育和行为过程中作用的一种成熟工具，但由于其手动操作和低通量的性质，其应用受到限制。在这里，我们展示了一种结合秀丽隐杆线虫的多重微流控操作和图像处理及自动化软件的技术，实现了高通量的细胞消融。