Shakoor Adnan, Gao Wendi, Zhao Libo, Jiang Zhuangde, Sun Dong
Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.
State Key Laboratory for Manufacturing Systems Engineering, International Joint Laboratory for Micro/Nano Manufacturing and Measurement Technologies, The School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, China.
Microsyst Nanoeng. 2022 Apr 29;8:47. doi: 10.1038/s41378-022-00376-0. eCollection 2022.
Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.
必须开发出能够操纵和研究细胞器及细胞成分的高精度微操纵工具，以支持新型细胞医学疗法的快速发展，从而促进对细胞动态、细胞成分功能和疾病机制的深入理解。本文对用于单细胞手术的微/纳米操纵工具及其控制方法进行了文献综述。详细介绍了具体基于激光、微针和无系留微/纳米工具的微操纵方法。还讨论了这些技术的局限性。本文还探讨了单细胞手术的生物学意义和临床应用。
