Department of Biology, Faculty of Arts and Sciences, Cukurova University, Adana 01330, Turkey.
Biol Trace Elem Res. 2010 Nov;137(2):235-43. doi: 10.1007/s12011-009-8573-9. Epub 2009 Dec 5.
The aim of the present study is to evaluate the oxidative effects of lead with increased concentrations by the determination of antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and ascorbate peroxidase (AP)) and lipid peroxidation levels in the stem and leaves of watercress (Nasturtium officinale R. Br.) which was exposed to lead acetate, Pb (CH3COOH)2 regime with concentrations of 0, 50, 100, 200, 250, and 500 mg/L Pb in a hydroponic culture. After 14 days, accumulation of lipid peroxidation in stems and leaves and changes in activity of antioxidant enzymes were determined spectrophotometrically. The maximum accumulation was observed in the highest concentration group. In this group, lipid peroxidation levels were three times higher than the control group in the stem and leaves. The highest induction in SOD and GR activities were determined at 200 mg/L Pb group in stem, whereas CAT and AP activities were higher than other groups at the concentration of 250 and 100 mg/L Pb, respectively. The increase in CAT activity was found to be greater than GR, SOD, and AP activities in stems of watercress under Pb treatment. Both lead accumulation and antioxidant enzyme responses were higher in stems than in leaves. The results of the present study suggested that the induction in antioxidant responses could be occurring as an adaptive mechanism to the oxidative potential of lead accumulation.
本研究旨在通过测定抗氧化酶活性（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和抗坏血酸过氧化物酶（AP））和水芹茎叶中脂质过氧化水平来评估铅浓度增加的氧化作用。水芹（ Nasturtium officinale R. Br.）在水培条件下暴露于醋酸铅 Pb（CH3COOH）2 处理，浓度分别为 0、50、100、200、250 和 500 mg/L Pb。14 天后，通过分光光度法测定茎和叶中脂质过氧化的积累和抗氧化酶活性的变化。在最高浓度组中观察到最大的积累。在该组中，茎和叶中的脂质过氧化水平比对照组高 3 倍。在茎中，SOD 和 GR 活性在 200 mg/L Pb 组中最高诱导，而 CAT 和 AP 活性在 250 和 100 mg/L Pb 浓度下分别高于其他组。在 Pb 处理下水芹茎中 CAT 活性的增加大于 GR、SOD 和 AP 活性。铅积累和抗氧化酶反应在茎中均高于叶片。本研究结果表明，抗氧化反应的诱导可能是作为对铅积累的氧化潜力的适应机制发生的。
