肌萎缩侧索硬化症患者脑脊液和血清中神经丝抗体。
Cerebrospinal fluid and serum antibodies against neurofilaments in patients with amyotrophic lateral sclerosis.
机构信息
First Faculty of Medicine, Institute of Medical Biochemistry, Charles University, Prague, Czech Republic.
出版信息
Eur J Neurol. 2010 Apr;17(4):562-6. doi: 10.1111/j.1468-1331.2009.02853.x. Epub 2009 Nov 24.
BACKGROUND
The aim of the study was to assess autoimmune involvement in amyotrophic lateral sclerosis (ALS).
METHODS
We measured IgG antibodies against light (NFL) and medium (NFM) subunits of neurofilaments using ELISA in paired cerebrospinal fluid (CSF) and serum samples from 38 ALS patients and 20 controls.
RESULTS
Serum levels of anti-NFL were higher in ALS patients than in controls (P < 0.005). Serum anti-NFL antibodies and intrathecal anti-NFM antibodies were related to patient disability (serum anti-NFL: P < 0.05; intrathecal anti-NFM: P < 0.05). Anti-NFL levels were significantly correlated with anti-NFM levels in ALS (P < 0.001) and the control group (P < 0.0001) in the CSF, but not in serum. Anti-NFL and anti-NFM antibodies significantly correlated between serum and CSF in the ALS group (anti-NFL: P < 0.0001; anti-NFM: P < 0.001) and in the control group (anti-NFL: P < 0.05; anti-NFM: P < 0.05).
CONCLUSIONS
Autoimmune humoral response to neurocytoskeletal proteins is associated with ALS.
背景
本研究旨在评估自身免疫在肌萎缩侧索硬化症（ALS）中的作用。
方法
我们使用 ELISA 法检测了 38 例 ALS 患者和 20 例对照者配对的脑脊液（CSF）和血清样本中神经丝轻（NFL）和中（NFM）亚单位的 IgG 抗体。
结果
与对照组相比，ALS 患者的血清抗-NFL 水平更高（P<0.005）。血清抗-NFL 抗体和鞘内抗-NFM 抗体与患者的残疾程度相关（血清抗-NFL：P<0.05；鞘内抗-NFM：P<0.05）。在 ALS 组（P<0.001）和对照组（P<0.0001）的 CSF 中，抗-NFL 水平与抗-NFM 水平显著相关，但在血清中则不相关。在 ALS 组（抗-NFL：P<0.0001；抗-NFM：P<0.001）和对照组（抗-NFL：P<0.05；抗-NFM：P<0.05）中，血清和 CSF 之间的抗-NFL 和抗-NFM 抗体均呈显著相关性。
结论
针对神经细胞骨架蛋白的自身免疫性体液反应与 ALS 相关。
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