Moritz Cesar Eduardo Jacintho, Teixeira Bruno Costa, Rockenbach Liliana, Reischak-Oliveira Alvaro, Casali Emerson André, Battastini Ana Maria Oliveira
Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciência do Movimento Humano, Escola de Educação Física, Universidade Federado do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Mol Cell Biochem. 2017 Feb;426(1-2):55-63. doi: 10.1007/s11010-016-2880-1. Epub 2016 Nov 16.
Nucleotidases participate in the regulation of physiological and pathological events, such as inflammation and coagulation. Exercise promotes distinct adaptations, and can influence purinergic signaling. In the present study, we investigated soluble nucleotidase activities in the blood serum of sedentary young male adults at pre- and post-acute moderate aerobic exercise. In addition, we evaluated how this kind of exercise could influence adenine nucleotide concentrations in the blood serum. Sedentary individuals were submitted to moderate aerobic exercise on a treadmill; blood samples were collected pre- and post-exercise, and serum was separated for analysis. Results showed increases in ATP, ADP, and AMP hydrolysis post-exercise, compared to pre-exercise values. The ecto-nucleotide pyrophosphatase/phosphodiesterase was also evaluated, showing an increased activity post-exercise, compared to pre-exercise. Purine levels were analyzed by HPLC in the blood serum, pre- and post-exercise. Decreased levels of ATP and ADP were found post-exercise, in contrast with pre-exercise values. Conversely, post-exercise levels of adenosine and inosine increased compared to pre-exercise levels. Our results indicate an influence of acute exercise on ATP metabolism, modifying enzymatic behavior to promote a protective biological environment.
核苷酸酶参与生理和病理过程的调节，如炎症和凝血。运动可促进不同的适应性变化，并能影响嘌呤能信号传导。在本研究中，我们调查了久坐不动的年轻男性成年人在急性中等强度有氧运动前后血清中的可溶性核苷酸酶活性。此外，我们评估了这种运动如何影响血清中的腺嘌呤核苷酸浓度。让久坐不动的个体在跑步机上进行中等强度有氧运动；在运动前后采集血样，并分离血清进行分析。结果显示，与运动前的值相比，运动后ATP、ADP和AMP的水解增加。还评估了胞外核苷酸焦磷酸酶/磷酸二酯酶，结果显示与运动前相比，运动后其活性增加。通过高效液相色谱法分析运动前后血清中的嘌呤水平。与运动前的值相比，运动后ATP和ADP水平降低。相反，与运动前水平相比，运动后腺苷和肌苷水平升高。我们的结果表明急性运动对ATP代谢有影响，改变酶的行为以促进保护性生物环境。
