蛋白结合丙烯醛与原发性干燥综合征的强相关性。
Intense correlation between protein-conjugated acrolein and primary Sjögren's syndrome.
机构信息
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
出版信息
Clin Chim Acta. 2010 Mar;411(5-6):359-63. doi: 10.1016/j.cca.2009.11.032. Epub 2009 Dec 5.
BACKGROUND
We recently found that an increased plasma concentration of protein-conjugated acrolein is a good biomarker for stroke. Therefore we determine whether the concentration of protein-conjugated acrolein is increased in saliva from patients with primary Sjögren's syndrome.
METHODS
Stimulated whole-mixed saliva was collected from 10 patients and 13 control subjects. The concentration of protein-conjugated acrolein in saliva and plasma was measured by either Western blotting or enzyme-linked immunosorbent assay.
RESULTS
The concentration of protein-conjugated acrolein, especially albumin-conjugated acrolein, was greatly increased in saliva from patients with primary Sjögren's syndrome (p<0.001). The concentration of protein-conjugated acrolein was inversely correlated with the flow rate of saliva.
CONCLUSION
The results indicate that the concentration of protein-conjugated acrolein, a marker of cell or tissue damage, in saliva is well correlated with seriousness of primary Sjögren's syndrome.
背景
我们最近发现，血浆中蛋白结合丙烯醛浓度的增加是中风的一个很好的生物标志物。因此，我们确定原发性干燥综合征患者唾液中是否存在蛋白结合丙烯醛浓度增加的情况。
方法
收集 10 名患者和 13 名对照者的刺激性全混合唾液。通过 Western blot 或酶联免疫吸附试验检测唾液和血浆中蛋白结合丙烯醛的浓度。
结果
原发性干燥综合征患者的唾液中蛋白结合丙烯醛浓度，特别是白蛋白结合丙烯醛浓度显著增加（p<0.001）。蛋白结合丙烯醛浓度与唾液流速呈负相关。
结论
这些结果表明，唾液中细胞或组织损伤标志物蛋白结合丙烯醛的浓度与原发性干燥综合征的严重程度密切相关。
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