Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):41-7. doi: 10.1016/j.cimid.2009.11.001. Epub 2009 Dec 6.
Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was detected initially adhered to and subsequently inside the host cells. Between 24 and 48 h of infection, Ms was detected in the perinuclear region, and after 72 h of infection was confirmed by gentamicin invasion assay. High and low passage Ms strains showed no differences in adherence or invasion. The morphology and the actin filaments of the infected HEp-2 cells were preserved throughout the study period. The observed invasion by Ms is consistent with the biology of Mollicutes, and could explain the difficulties in recovering field isolates of the mycoplasma and in controlling the infection in birds even after long-term antibiotic treatment.
将两种田间分离株和滑液支原体（Ms）的荧光标记细胞接种到荧光标记的 HEp-2 细胞单层培养物上，并通过共聚焦激光扫描显微镜（CLSM）进行监测。最初观察到 Ms 附着在宿主细胞上，随后进入宿主细胞内部。在感染后 24 至 48 小时，Ms 被检测到在核周区域，并且在感染 72 小时后通过庆大霉素侵袭试验得到确认。高传代和低传代 Ms 株在黏附和侵袭方面没有差异。在整个研究期间，感染的 HEp-2 细胞的形态和肌动蛋白丝保持不变。观察到的 Ms 侵袭与柔膜体纲的生物学特性一致，这可以解释为什么即使经过长期抗生素治疗，也难以从田间分离株中回收支原体并控制鸟类感染。
