STD-DOSY：一种分析多组分酶/底物体系的新 NMR 方法。

STD-DOSY: A new NMR method to analyze multi-component enzyme/substrate systems.

机构信息

University of Potsdam, Department of Chemistry, Karl-Liebknecht-Str. 24-25, 14476 Potsdam/Golm, Germany.

出版信息

J Magn Reson. 2010 Feb;202(2):245-9. doi: 10.1016/j.jmr.2009.11.007. Epub 2009 Dec 6.

DOI:10.1016/j.jmr.2009.11.007

PMID:19969494

Abstract

A new approach to analyze multi-component Saturation Transfer Difference (STD) NMR spectra by combining the STD and the DOSY experiment is proposed. The resulting pulse sequence was successfully used to simplify an exemplary multi-component protein/substrate system by means of standard DOSY processing methods. Furthermore, the same experiment could be applied to calculate the ratio of saturated substrate molecules and its saturation rate in the case of competitive interactions. This ratio depends on the strength of this interaction between the substrates and the protein, so that this kind of information could be extracted from the results of our experiment.

摘要

提出了一种通过结合 STD 和 DOSY 实验来分析多分量饱和转移差异（STD）NMR 谱的新方法。所得到的脉冲序列成功地用于简化示例多分量蛋白质/底物系统，方法是使用标准 DOSY 处理方法。此外，相同的实验可以应用于计算在竞争相互作用的情况下饱和底物分子的比例及其饱和速率。该比例取决于底物与蛋白质之间相互作用的强度，因此可以从我们的实验结果中提取此类信息。