Centro de Investigaciones Biologicas-CSIC. Calle Ramiro de Maeztu, 9. 28040. Madrid, Spain.
Curr Protein Pept Sci. 2012 Dec;13(8):816-30. doi: 10.2174/138920312804871175.
Diseases that result from infection are, in general, a consequence of specific interactions between a pathogenic organism and the cells. The study of host-pathogen interactions has provided insights for the design of drugs with therapeutic properties. One area that has proved to be promising for such studies is the constituted by carbohydrates which participate in biological processes of paramount importance. On the one hand, carbohydrates have shown to be information carriers with similar, if not higher, importance than traditionally considered carriers as amino acids and nucleic acids. On the other hand, the knowledge on molecular recognition of sugars by lectins and other carbohydrate-binding proteins has been employed for the development of new biomedical strategies. Biophysical techniques such as X-Ray crystallography and NMR spectroscopy lead currently the investigation on this field. In this review, a description of traditional and novel NMR methodologies employed in the study of sugar-protein interactions is briefly presented in combination with a palette of NMR-based studies related to biologically and/or pharmaceutically relevant applications.
由感染引起的疾病通常是致病生物体与细胞之间特定相互作用的结果。宿主-病原体相互作用的研究为具有治疗特性的药物设计提供了思路。已经证明，在这样的研究中，有一个领域具有很大的发展潜力，那就是由碳水化合物组成的领域，这些碳水化合物参与了至关重要的生物过程。一方面，碳水化合物已经被证明是信息载体，其重要性与传统上被认为的载体（如氨基酸和核酸）相似，如果不是更高的话。另一方面，糖与凝集素和其他碳水化合物结合蛋白的分子识别的知识已被用于开发新的生物医学策略。目前，X 射线晶体学和 NMR 光谱学等生物物理技术引领着这一领域的研究。在这篇综述中，简要介绍了用于研究糖-蛋白相互作用的传统和新型 NMR 方法，并结合了一系列基于 NMR 的与生物和/或药物相关的应用研究。
