通过NOE和交换相互作用探测福斯曼抗原的溶液构象。

Solution conformation of Forssman antigen probed by NOE and exchange interactions.

作者信息

Poppe L, Dabrowski J, von der Lieth C W

机构信息

Max-Planck-Institut für Medizinische Forschung, Heidelberg, Germany.

出版信息

Biochem Biophys Res Commun. 1991 Feb 14;174(3):1169-75. doi: 10.1016/0006-291x(91)91544-m.

DOI:10.1016/0006-291x(91)91544-m

PMID:1996984

Abstract

The conformation of Forssman glycolipid, GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1ceramide, was analysed with the aid of the rotating frame NOE and Hartmann-Hahn spectroscopy. NOE contacts between C-, O-, and N-linked protons were used for distance mapping. The glycosidic bonds that are common to globotriaosylceramide and globoside showed a similar flexibility as found for these compounds [Poppe et al., (1990) Eur. J. Biochem. 189, 313-325; J. Am. Chem. Soc. 112, 7762-7771]. In contrast, the conformational mobility of the terminal GalNAc alpha 1-3GalNAc beta linkage appears to be restrained. A new approach, based on 2D exchange spectroscopy, was proposed for revealing of spatial proximities between exchangeable protons in Me2SO solution.

摘要

借助旋转坐标系NOE和Hartmann-Hahn光谱对福斯曼糖脂（GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1神经酰胺）的构象进行了分析。C-、O-和N-连接质子之间的NOE接触用于距离映射。与球三糖神经酰胺和球苷共有的糖苷键表现出与这些化合物相似的灵活性[Poppe等人，（1990年）《欧洲生物化学杂志》189卷，313 - 325页；《美国化学会志》112卷，7762 - 7771页]。相比之下，末端GalNAcα1-3GalNAcβ连接的构象流动性似乎受到限制。提出了一种基于二维交换光谱的新方法，用于揭示Me2SO溶液中可交换质子之间的空间接近性。