Thompson H L, Burbelo P D, Yamada Y, Kleinman H K, Metcalfe D D
Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Immunology. 1991 Jan;72(1):144-9.
PT18 mast cells and mouse bone marrow-derived mast cells have been shown to adhere and spread when in contact with a laminin substratum. Mouse bone marrow cells, however, first require activation with phorbol myristate acetate (PMA), ionophore, or antigen-specific IgE with antigen in order to exhibit these phenomena. Here, we have studied the interaction of these cells with three active synthetic peptides derived from different domains of laminin. PT18 cells and mouse bone marrow mast cells attached and spread on the 19 amino acid synthetic laminin A chain-derived peptide PA22-2, containing the active five amino acid sequence IKVAV, and this attachment did not require prior activation of the mouse bone marrow mast cells with PMA or IgE plus antigen. These cells did not adhere to the B1 chain peptide YIGSR-NH2 or the RGD-containing peptide from the A chain. PT18 cell adherence to laminin was inhibited by soluble peptide PA22-2, but not by either YIGSR-NH2, the RGD-containing, or control peptides. Antisera to the PA22-2 peptide completely abolished adherence to PA22-2, but only partially inhibited mast cell adherence to laminin. Antibody to the 67,000-32,000 MW laminin-binding protein receptor blocked cell adhesion to laminin and to the active A chain peptide. Thus, mast cell adhesion and spreading on laminin may be mediated by an interaction with the IKVAV sequence on the laminin A chain.
已表明，PT18肥大细胞和小鼠骨髓来源的肥大细胞在与层粘连蛋白基质接触时会发生黏附并铺展。然而，小鼠骨髓细胞首先需要用佛波醇肉豆蔻酸酯乙酸酯（PMA）、离子载体或抗原特异性IgE与抗原进行激活，才能表现出这些现象。在此，我们研究了这些细胞与源自层粘连蛋白不同结构域的三种活性合成肽的相互作用。PT18细胞和小鼠骨髓肥大细胞在含有活性五肽序列IKVAV的19个氨基酸的合成层粘连蛋白A链衍生肽PA22 - 2上附着并铺展，并且这种附着不需要事先用PMA或IgE加抗原激活小鼠骨髓肥大细胞。这些细胞不黏附于B1链肽YIGSR - NH2或A链的含RGD肽。PT18细胞对层粘连蛋白的黏附被可溶性肽PA22 - 2抑制，但不被YIGSR - NH2、含RGD肽或对照肽抑制。针对PA22 - 2肽的抗血清完全消除了对PA22 - 2的黏附，但仅部分抑制肥大细胞对层粘连蛋白的黏附。针对67,000 - 32,000 MW层粘连蛋白结合蛋白受体的抗体阻断了细胞对层粘连蛋白和活性A链肽的黏附。因此，肥大细胞在层粘连蛋白上的黏附和铺展可能是由与层粘连蛋白A链上的IKVAV序列相互作用介导的。
