Thompson H L, Burbelo P D, Segui-Real B, Yamada Y, Metcalfe D D
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
J Immunol. 1989 Oct 1;143(7):2323-7.
Tissue mast cells often localize in close proximity to the basement membrane of endothelial cells and increase at sites of inflammation. The reason for this unique tissue distribution is unknown. We report here that both the murine mast cell line PT18 and mouse bone marrow-derived mast cells possess functional receptors for laminin, and exhibit adhesion, spreading and redistribution of histamine-containing granules on a laminin substratum. This adherence is enhanced in the presence of purified IL-3 and can be inhibited by antibodies to laminin and by antibodies to laminin receptors. Northern analysis showed a high level of mRNA for a 32-kDa laminin receptor in PT18 mast cells. Mouse bone marrow-derived cultures initially exhibited a low level of the mRNA expression. However, the expression of the laminin receptor mRNA is induced rapidly within 1 wk of culture with IL-3. Thus, mast cells exhibit functional laminin receptors that may explain the tissue distribution of mast cells and their accumulation at sites of tissue injury.
组织肥大细胞常定位于内皮细胞基底膜附近，并在炎症部位增多。这种独特的组织分布原因尚不清楚。我们在此报告，小鼠肥大细胞系PT18和小鼠骨髓来源的肥大细胞都具有层粘连蛋白的功能性受体，并在层粘连蛋白基质上表现出粘附、铺展以及含组胺颗粒的重新分布。在纯化的白细胞介素-3存在下，这种粘附作用增强，并且可被层粘连蛋白抗体和层粘连蛋白受体抗体抑制。Northern分析显示PT18肥大细胞中32 kDa层粘连蛋白受体的mRNA水平很高。小鼠骨髓来源的培养物最初显示出较低水平的mRNA表达。然而，在用白细胞介素-3培养1周内，层粘连蛋白受体mRNA的表达迅速被诱导。因此，肥大细胞表现出功能性层粘连蛋白受体，这可能解释了肥大细胞的组织分布及其在组织损伤部位的积聚。
