Thompson H L, Burbelo P D, Yamada Y, Kleinman H K, Metcalfe D D
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
J Immunol. 1989 Dec 15;143(12):4188-92.
The increase of mast cells at sites of tissue inflammation suggests the production of local factors chemotactic for mast cells. In this report, we demonstrate that the murine mast cell line PT18 and primary mouse bone marrow-derived mast cells chemotax to the basement membrane glycoprotein laminin, and that the synthetic laminin A chain-derived peptide, PA22-2, represents a region of laminin that contains a major chemoattractant site. Mast cell chemotaxis to laminin is enhanced after activation of mast cells by the calcium ionophore, A23187, or PMA and by sensitization of the cells with IgE followed by exposure to antigen. Chemotaxis is not increased in the presence of IL-3 and is independent of mast cell degranulation, as histamine release did not occur when cells were activated with PMA. Mast cell chemotaxis to laminin and its enhancement by IgE-dependent mast cell activation provides a mechanism by which these cells may be attracted to sites of tissue injury. Such activity may be particularly relevant in the response of host tissues to inflammation accompanying parasitic infestations, allergic reactions, and wound healing.
组织炎症部位肥大细胞数量的增加提示存在对肥大细胞具有趋化作用的局部因子。在本报告中，我们证明小鼠肥大细胞系PT18和原代小鼠骨髓来源的肥大细胞对基底膜糖蛋白层粘连蛋白具有趋化作用，并且合成的层粘连蛋白A链衍生肽PA22 - 2代表层粘连蛋白中包含主要趋化位点的区域。在用钙离子载体A23187或PMA激活肥大细胞后，以及在用IgE致敏细胞后再暴露于抗原，肥大细胞对层粘连蛋白的趋化作用增强。在存在IL - 3的情况下趋化作用不增加，并且与肥大细胞脱颗粒无关，因为在用PMA激活细胞时未发生组胺释放。肥大细胞对层粘连蛋白的趋化作用及其通过IgE依赖的肥大细胞激活而增强，为这些细胞被吸引到组织损伤部位提供了一种机制。这种活性在宿主组织对伴随寄生虫感染、过敏反应和伤口愈合的炎症反应中可能特别重要。
