Culvenor J G, Day K P, Anders R F
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Infect Immun. 1991 Mar;59(3):1183-7. doi: 10.1128/iai.59.3.1183-1187.1991.
Electron microscopy was used to study the fate of Plasmodium falciparum ring-infected erythrocyte surface antigen after merozoite invasion by using postembedding immunolabeling. The antigen was localized to small dense granules located centrally or laterally in free merozoites. In newly invaded erythrocytes, labeling was found in pockets of the parasitophorous vacuole space or in aggregates closely associated with the parasitophorous vacuole. These patterns indicate that ring-infected erythrocyte surface antigen is contained in merozoite dense granules that are released after merozoite invasion and not via apical rhoptry ducts at the time of merozoite attachment.
利用包埋后免疫标记，通过电子显微镜研究恶性疟原虫环状体感染红细胞表面抗原在裂殖子侵入后的命运。该抗原定位于游离裂殖子中央或侧面的小致密颗粒中。在新侵入的红细胞中，在寄生泡空间的小窝或与寄生泡紧密相关的聚集体中发现有标记。这些模式表明，环状体感染红细胞表面抗原包含在裂殖子致密颗粒中，这些颗粒在裂殖子侵入后释放，而不是在裂殖子附着时通过顶端的棒状体导管释放。
