Uni S, Masuda A, Stewart M J, Igarashi I, Nussenzweig R, Aikawa M
Am J Trop Med Hyg. 1987 May;36(3):481-8. doi: 10.4269/ajtmh.1987.36.481.
The subcellular localization of the 150/130 Kd antigen in Plasmodium falciparum-infected erythrocytes was determined by electron microscopy using monoclonal antibody 9B11 and immuno-gold labeling. We now find that this antigen may be associated with the membrane of newly-infected human erythrocytes and the cytoplasm of ring stage parasites. During differentiation of the parasite to the trophozoite stage, the antigens are no longer detectable on the erythrocyte membrane, while gold particles become more numerous within the parasite and in the erythrocyte cytoplasm adjacent to the parasite. As the parasites develop into schizonts, more antigen appears within the parasites, and some of it appears in the erythrocyte cytoplasm. At the segmented schizont stage, many intraparasitic gold particles are associated with rhoptries and micronemes of developing merozoites. Likewise, gold particles are associated with elements of the rhoptry-microneme complex in free merozoites. No gold particles are detected on the surface of merozoites. These antigens are found most abundantly in erythrocytes infected with gametocytes, revealing a localization pattern similar to that of mature trophozoite-infected erythrocytes. These subcellular localization patterns are similar to those described for the ring-infected erythrocyte surface antigen.
利用单克隆抗体9B11和免疫金标记，通过电子显微镜确定了恶性疟原虫感染红细胞中150/130 Kd抗原的亚细胞定位。我们现在发现，这种抗原可能与新感染的人类红细胞膜以及环状体期疟原虫的细胞质有关。在疟原虫分化为滋养体期的过程中，红细胞膜上不再能检测到该抗原，而在疟原虫内以及与疟原虫相邻的红细胞细胞质中，金颗粒变得更加密集。随着疟原虫发育成裂殖体，更多的抗原出现在疟原虫内，并且其中一些出现在红细胞细胞质中。在裂殖体分段期，许多寄生体内的金颗粒与发育中的裂殖子的棒状体和微线体有关。同样，金颗粒也与游离裂殖子中的棒状体-微线体复合体成分有关。在裂殖子表面未检测到金颗粒。这些抗原在感染配子体的红细胞中含量最为丰富，显示出与感染成熟滋养体的红细胞相似的定位模式。这些亚细胞定位模式与环状体感染红细胞表面抗原所描述的模式相似。
