Ammigan N, Nair U J, Lalitha V S, Bhide S V
Carcinigenesis Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
J Cancer Res Clin Oncol. 1991;117(1):50-4. doi: 10.1007/BF01613196.
The carcinogenicity of long-term feeding of masheri extract to animals in a vitamin-A-sufficient (SLO+) and deficient (SLO-) state was studied in Sprague Dawley rats by feeding daily dose of 3 mg extract over a period of 21 months. The phase I activating enzymes, the glutathione (GSH)/glutathione S-transferase (GST) detoxification system, and the hepatic and circulating levels of vitamins A and C were also monitored at 12 and 21 months. It was observed that the phase I enzyme activities were significantly higher in SLO+ than in SLO- rats at both 12 months and 21 months. Moreover, the SLO- masheri-treated animals also showed a decreased in the GSH/GST detoxification system while the reverse was observed in SLO+ group. Masheri extract treatment significantly lowered the hepatic and circulating levels of vitamin A while a concurrent increase was observed in the vitamin C level. The extract was found to be tumorigenic in both the SLO+ and SLO- groups. Benign tumours were observed in the SLO+ group while a high incidence of malignant tumours of the lung were observed in the SLO- group upon treatment with masheri extract.
通过在21个月的时间里每天给斯普拉格-道利大鼠喂食3毫克马舍里提取物，研究了在维生素A充足（SLO+）和缺乏（SLO-）状态下长期给动物喂食马舍里提取物的致癌性。在12个月和21个月时，还监测了I期激活酶、谷胱甘肽（GSH）/谷胱甘肽S-转移酶（GST）解毒系统以及肝脏和循环中维生素A和C的水平。观察到，在12个月和21个月时，SLO+大鼠的I期酶活性显著高于SLO-大鼠。此外，经马舍里提取物处理的SLO-动物的GSH/GST解毒系统也有所下降，而在SLO+组中观察到相反的情况。马舍里提取物处理显著降低了肝脏和循环中维生素A的水平，同时维生素C水平出现了相应升高。发现该提取物在SLO+和SLO-组中均具有致瘤性。在用马舍里提取物处理后，SLO+组中观察到良性肿瘤，而SLO-组中观察到肺部恶性肿瘤的高发病率。
