Handa A, Hoshino H, Nakajima K, Adachi M, Ikeda K, Achiwa K, Itoh T, Suzuki Y
Department of Hygiene, Gunma University School of Medicine, Japan.
Biochem Biophys Res Commun. 1991 Feb 28;175(1):1-9. doi: 10.1016/s0006-291x(05)81191-x.
Four kinds of gangliosides, namely GM1a, GD1a, GD1b and GT1b and their sulfated derivatives were examined for antiviral activities against human immunodeficiency virus type 1 and abilities to modulate CD4 antigen on the cell surface. The infection of human T cells with the virus was markedly inhibited by treatment with the sulfated gangliosides at a concentration of 10 micrograms/ml, while the non-sulfated gangliosides had only weak antiviral activities. The sulfated gangliosides completely inhibited syncytium formation induced by HIV-1 at 30 micrograms/ml. The CD4 antigen on the surface of T cells became hardly detectable after treatment with them. They did not damage cells, nor prolong the activated partial thromboplastin time at concentrations of up to 100 micrograms/ml, suggesting that they may have little side effect in vivo.
研究了四种神经节苷脂，即GM1a、GD1a、GD1b和GT1b及其硫酸化衍生物对1型人类免疫缺陷病毒的抗病毒活性以及调节细胞表面CD4抗原的能力。浓度为10微克/毫升的硫酸化神经节苷脂处理可显著抑制人T细胞被该病毒感染，而非硫酸化神经节苷脂的抗病毒活性较弱。硫酸化神经节苷脂在30微克/毫升时可完全抑制HIV-1诱导的合胞体形成。用它们处理后，T细胞表面的CD4抗原几乎无法检测到。在浓度高达100微克/毫升时，它们不会损伤细胞，也不会延长活化部分凝血活酶时间，这表明它们在体内可能几乎没有副作用。
