一种用于人碱性成纤维细胞生长因子的灵敏酶免疫测定法。

A sensitive enzyme immunoassay for human basic fibroblast growth factor.

作者信息

Watanabe H, Hori A, Seno M, Kozai Y, Igarashi K, Ichimori Y, Kondo K

机构信息

Chemistry Research Laboratory, Takeda Chemical Industries Ltd., Osaka, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Feb 28;175(1):229-35. doi: 10.1016/s0006-291x(05)81224-0.

DOI:10.1016/s0006-291x(05)81224-0

PMID:1998507

Abstract

A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neither human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30 approximately 206 pg/ml.

摘要

利用三种单克隆抗体（MAb3H3、MAb98和MAb52）开发了一种用于人碱性成纤维细胞生长因子（HbFGF）的灵敏夹心酶免疫测定法。抑制HbFGF生物活性的MAb3H3的Fab'片段与辣根过氧化物酶偶联。固相使用MAb52和MAb98的混合物。在该测定系统中，人酸性成纤维细胞生长因子、hst-1/KS3产物或酸变性的HbFGF均无交叉反应。该测定系统的检测限为1 pg/孔。使用该测定法，发现一些肿瘤细胞系产生的bFGF水平高于正常细胞系。还对正常志愿者的血清样本进行了检测，在57个样本中的16个样本中检测到免疫反应性HbFGF，范围为30至约206 pg/ml。