Orekhov A N, Tertov V V, Kabakov A E, Pokrovsky S N, Smirnov V N
Institute of Experimental Cardiology, U.S.S.R. Cardiology Research Center, Moscow.
Arterioscler Thromb. 1991 Mar-Apr;11(2):316-26. doi: 10.1161/01.atv.11.2.316.
The blood serum of patients with coronary atherosclerosis possesses an ability to induce the accumulation of cellular lipids in primary cultures of human aortic intimal cells. Factors responsible for this property of the atherosclerotic patients' sera are represented by modified (desialylated) low density lipoprotein (LDL) and a nonlipid factor interacting with LDL. It was assumed that the nonlipid factor was antibodies against LDL. Total immunoglobulin G (IgG) fraction was isolated from the sera of atherosclerotic patients, and IgGs interacting with LDL (anti-LDL) were then purified by affinity chromatography on a sorbent with immobilized LDL. From the sera of patients, a 30-fold greater amount of anti-LDL has been isolated than from the sera of healthy donors. The affinity constant of anti-LDL to the lipoprotein obtained from the blood of healthy donors was 2 x 10(7) M-1. The affinity of anti-LDL to the lipoprotein from the blood of atherosclerotic patients, as well as to LDL desialylated in vitro with neuraminidase, was much higher. Anti-LDL increased the uptake of LDL by cultured aortic cells by approximately 2.5-fold and substantially increased intracellular lipid accumulation. The obtained data suggest that autoantibodies against LDL are an essential factor of blood plasma responsible for its atherogenic potential.
冠状动脉粥样硬化患者的血清具有诱导人主动脉内膜细胞原代培养物中细胞脂质积累的能力。动脉粥样硬化患者血清的这种特性所涉及的因素是修饰的（去唾液酸化的）低密度脂蛋白（LDL）和一种与LDL相互作用的非脂质因子。据推测，该非脂质因子是抗LDL抗体。从动脉粥样硬化患者的血清中分离出总免疫球蛋白G（IgG）组分，然后通过在固定有LDL的吸附剂上进行亲和层析纯化与LDL相互作用的IgG（抗LDL）。从患者血清中分离出的抗LDL量比健康供体血清中多30倍。抗LDL与从健康供体血液中获得的脂蛋白的亲和常数为2×10⁷ M⁻¹。抗LDL与动脉粥样硬化患者血液中的脂蛋白以及与用神经氨酸酶在体外去唾液酸化的LDL的亲和力要高得多。抗LDL使培养的主动脉细胞对LDL的摄取增加约2.5倍，并显著增加细胞内脂质积累。所获得的数据表明，抗LDL自身抗体是血浆中导致其动脉粥样硬化潜力的一个重要因素。
