Séguin C
Centre de Recherche en Cancérologie de l'Université Laval, L'Hôtel-Dieu de Québec, Canada.
Gene. 1991 Jan 15;97(2):295-300. doi: 10.1016/0378-1119(91)90066-k.
The metal ion requirement of nuclear proteins for binding to the metal regulatory element d(MREd) of the mouse gene encoding metallothionein-1 was investigated using an in vitro exonuclease III footprinting assay. The specific DNA-binding activity of the factor was inactivated by the chelating agents, EDTA and 1,10-phenanthroline. Binding activity was restored by Zn2+, but not by Cd2+. These results show that Zn2+ ions are a required component for specific in vitro DNA binding of the MREd-binding protein.
利用体外核酸外切酶III足迹分析，研究了核蛋白与编码金属硫蛋白-1的小鼠基因的金属调节元件d（MREd）结合时对金属离子的需求。螯合剂乙二胺四乙酸（EDTA）和1,10-菲咯啉使该因子的特异性DNA结合活性失活。锌离子（Zn2+）可恢复结合活性，而镉离子（Cd2+）则不能。这些结果表明，锌离子是MREd结合蛋白在体外特异性结合DNA所需的成分。
