Department of Buco-Maxilo-Facial Surgery and Periodontology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
J Clin Periodontol. 2010 Feb;37(2):180-90. doi: 10.1111/j.1600-051X.2009.01512.x. Epub 2009 Dec 7.
This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy.
GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically.
At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p<0.001), and these molecules decreased after therapy (p<0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p<0.01).
Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.
本研究旨在比较慢性牙周炎（CP）患者和对照组在基线和非手术治疗后 3 个月时龈沟液（GCF）中基质金属蛋白酶（MMP）-8、基质金属蛋白酶抑制剂（TIMP）-1 和 TIMP-2、髓过氧化物酶（MPO）和 MMP-9 的水平。
从 15 名对照组和 27 名 CP 患者的一个部位采集 GCF。通过酶联免疫吸附试验测定 MMP-8、MMP-9、TIMP-1 和 TIMP-2；通过明胶酶谱法测定不同形式的 MMP-9；通过比色法测定 MPO。
基线时，与对照组相比，患者的 MMP-8、TIMP-2、MPO 和 87 kDa-MMP-9 水平更高（p<0.001），治疗后这些分子水平下降（p<0.03）。两组之间 MMP-9 的高分子形式（180、130、92 kDa）或总 MMP-9 在基线时无差异。TIMP-1 水平无差异。在对照组中，与基线相比，治疗 3 个月后 TIMP-2 和 MMP-9 的高分子形式（180、130、92 kDa）水平降低（p<0.01）。
与对照组相比，患者 GCF 中 MMP-8、TIMP-2、MPO 和 87 kDa-MMP-9 水平更高，牙周治疗 3 个月后这些标志物水平下降。
