Key Lab of Education Ministry of China in Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Wuhan 430070, PR China.
Vaccine. 2010 Feb 10;28(6):1541-6. doi: 10.1016/j.vaccine.2009.11.058. Epub 2009 Dec 6.
The aim of current study was to evaluate the prospects of adjuvants against DNA vaccination (pES/2SS) encoding somatostatin (SS) and hepatitis B surface antigen fusion gene. A total of 60 female Hu lambs were divided into 6 groups and vaccinated in the context of various adjuvants (and controls): pE-CpG, Escherichia coli DH5alpha DNA, crude liposomes or GM-CSF in combination with the pES/2SS plasmid. Controls included pES/2SS only vaccinated and physiological saline groups. The antibody against SS level in the E. coli DH5alpha DNA group was significantly increased compared to that in the pES/2SS vaccine alone. Vaccination with pES/2SS/pE-CpG or pES/2SS/E. coli DH5alpha resulted in elevated weight gains that were 33.0 and 31.6% higher, respectively, than in saline group and pES/2SS only vaccinated controls. The concentrations of GH and IGF-I in the DNA vaccine groups were remarkably higher than those in the saline group, and those with positive antibody higher than negative antibody. These results suggested that different adjuvant/pES/2SS combinations can enhance the immune effect and had significant positive effects on growth.
本研究旨在评估佐剂对含有生长抑素（SS）和乙肝表面抗原融合基因的 DNA 疫苗（pES/2SS）的应用前景。将 60 只雌性湖羊分为 6 组，分别用不同佐剂（及对照）进行免疫：pE-CpG、大肠杆菌 DH5α 质粒、粗制脂质体或 GM-CSF 联合 pES/2SS 质粒。对照组包括单独接种 pES/2SS 质粒和生理盐水组。与单独接种 pES/2SS 组相比，大肠杆菌 DH5α 质粒组 SS 抗体水平显著升高。pES/2SS/pE-CpG 或 pES/2SS/大肠杆菌 DH5α 免疫组的体重增加分别比生理盐水组和单独接种 pES/2SS 对照组高 33.0%和 31.6%。DNA 疫苗组的 GH 和 IGF-I 浓度明显高于生理盐水组，且抗体阳性组高于抗体阴性组。这些结果表明，不同佐剂/pES/2SS 联合使用可以增强免疫效果，对生长有显著的积极影响。
