尿总β-N-乙酰-β-D-氨基葡萄糖苷酶和B型β-N-乙酰-β-D-氨基葡萄糖苷酶的半自动荧光测定法：分析研究

Semi-automated fluorometric assay for urinary total and B N-acetyl-beta-D-glucosaminidase: analytical investigation.

作者信息

Kristz W, Samaan G J, Leberre C, Demelier J F, Biou D

机构信息

Service de Biochimie-Hormonologie, Hôpital Robert Debre, Paris, France.

出版信息

J Clin Lab Anal. 1991;5(1):1-2. doi: 10.1002/jcla.1860050102.

DOI:10.1002/jcla.1860050102

PMID:1999757

Abstract

Total N-acetyl-beta-D-glucosaminidase (NAG) and NAG-B isoenzyme determination are determined in order to investigate renal tubular function. Here, we propose a semi-automated adaptation of Sandman's manual method for the Monarch centrifugal analyzer. Fluorescence of the released 4-methylumbelliferone is automatically read and results are directly expressed in nanokatal/l. Mean (1 SD) values expressed in nanokatal/millimole creatinine in urine from healthy female (n = 30) and male (n = 30) subjects were 8.2 (4.0) and 7.8 (2.9) for total NAG and 1.9 (1.4) and 1.5 (0.7) for the NAG-B isoenzyme activity. The assay of six patients with various renal disorders shows definite increase in total NAG and NAG-B isoenzyme activities.

摘要

为研究肾小管功能，进行了总N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAG）和NAG - B同工酶的测定。在此，我们提出一种针对Monarch离心分析仪的Sandman手工方法的半自动改进方法。自动读取释放的4 - 甲基伞形酮的荧光，结果直接以纳摩尔/升表示。健康女性（n = 30）和男性（n = 30）受试者尿液中以纳摩尔/毫摩尔肌酐表示的总NAG的平均值（1个标准差）分别为8.2（4.0）和7.8（2.9），NAG - B同工酶活性分别为1.9（1.4）和1.5（0.7）。对六名患有各种肾脏疾病的患者进行的检测显示，总NAG和NAG - B同工酶活性明显增加。