Morino Tsunetaro, Kikuchi Shun, Inagaki Taro, Komori Manabu, Yamamoto Kazuhisa, Kojima Hiromi, Yamato Masayuki
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Regen Ther. 2020 May 16;14:296-298. doi: 10.1016/j.reth.2020.03.003. eCollection 2020 Jun.
Experimental rabbits provide evidence for translational research regarding the pathogenies or treatment of human diseases. We developed a novel method for regenerating the middle ear mucosa using autologous cultured nasal mucosal epithelial cell sheets, and evaluated the wound healing process in the middle ear mucosa of experimental rabbits. Nonetheless, vigilant microbiological monitoring of experimental animals is essential to effectively prevent a decline in their health conditions, which may affect the research results. We experimented with contamination of in non-specific-pathogen-free (SPF) rabbits (without microbiological monitoring). Most non-SPF rabbits had otitis media, whereas SPF rabbits did not, which affected their results during the mucosal regeneration study. The contamination was resolved by changing the experimental design from using non-SPF rabbits to that using SPF rabbits. It is crucial to use the SPF animals for any surgical intervention studies.
实验兔为人类疾病的发病机制或治疗的转化研究提供了证据。我们开发了一种使用自体培养的鼻黏膜上皮细胞片再生中耳黏膜的新方法，并评估了实验兔中耳黏膜的伤口愈合过程。尽管如此，对实验动物进行严格的微生物监测对于有效防止其健康状况下降至关重要，因为这可能会影响研究结果。我们在无特定病原体（SPF）兔（未进行微生物监测）中进行了污染实验。大多数非SPF兔患有中耳炎，而SPF兔没有，这影响了它们在黏膜再生研究中的结果。通过将实验设计从使用非SPF兔改为使用SPF兔，污染问题得到了解决。对于任何手术干预研究，使用SPF动物至关重要。
