Centre for Environmental Risk Assessment and Remediation, Division of Information Technology, Engineering and the Environment, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095 Australia.
Environ Sci Technol. 2009 Dec 15;43(24):9487-94. doi: 10.1021/es902427y.
Currently, a number of in vitro methods are in use worldwide to assess arsenic (As) bioaccessibility in soils. However, a dearth of research has been undertaken to compare the efficacy of the in vitro methods for estimating in vivo relative As bioavailability. In this study, As bioaccessibility in contaminated soils (n = 12) was assessed using four in vitro assays (SBRC, IVG, PBET, DIN). In vitro results were compared to in vivo relative As bioavailability data (swine assay) to ascertain which methodologies best correlate with in vivo data. Arsenic bioaccessibility in contaminated soils varied depending on the in vitro method employed. For the SBRC and IVG methods, As bioaccessibility generally decreased when gastric-phase values were compared to the intestinal phase. In contrast, extending the PBET and DIN assays from the gastric to the intestinal phase resulted in an increase in As bioaccessibility for some soils tested. Comparison of in vitro and in vivo results demonstrated that the in vitro assay encompassing the SBRC gastric phase provided the best prediction of in vivo relative As bioavailability (R(2) = 0.75, Pearson correlation = 0.87). However, relative As bioavailability could also be predicted using gastric or intestinal phases of IVG, PBET, and DIN assays but with varying degrees of confidence (R(2) = 0.53-0.67, Pearson correlation = 0.73-0.82).
目前，全球范围内有许多用于评估土壤中砷（As）生物可利用性的体外方法。然而，对于比较体外方法评估体内相对 As 生物可利用性的效果的研究却很少。在这项研究中，使用四种体外测定法（SBRC、IVG、PBET、DIN）评估了污染土壤中的 As 生物可利用性（n=12）。将体外结果与体内相对 As 生物可利用性数据（猪测定）进行比较，以确定哪种方法与体内数据相关性最好。受污染土壤中的 As 生物可利用性取决于所采用的体外方法。对于 SBRC 和 IVG 方法，当比较胃相值与肠相值时，As 生物可利用性通常会降低。相比之下，将 PBET 和 DIN 测定法从胃相扩展到肠相，会导致一些测试土壤的 As 生物可利用性增加。体外和体内结果的比较表明，包含 SBRC 胃相的体外测定法最能预测体内相对 As 生物可利用性（R²=0.75，皮尔逊相关系数=0.87）。然而，也可以使用 IVG、PBET 和 DIN 测定法的胃相或肠相来预测相对 As 生物可利用性，但置信度不同（R²=0.53-0.67，皮尔逊相关系数=0.73-0.82）。
