INRA, PAPPSO (Plate-Forme d'Analyse Protéomique de Paris Sud-Ouest), Jouy en Josas, France.
J Proteome Res. 2010 Feb 5;9(2):677-88. doi: 10.1021/pr9000866.
We characterized the insoluble proteome of Lactococcus lactis using 1D electrophoresis-LC-MS/MS and identified 313 proteins with at least two different peptides. The identified proteins include 89 proteins having a predicted signal peptide and 25 predicted to be membrane-located. In addition, 67 proteins had alkaline isoelectric point values. Using spectra and peptide counts, we compared protein abundances in two different conditions: growth in rich medium, and after transit in the mouse digestive tract. We identified the large mechanosensitive channel and a putative cation transporter as membrane markers of bacterial adaptation to the digestive tract.
我们使用 1D 电泳-LC-MS/MS 对乳球菌的不可溶性蛋白质组进行了表征，共鉴定出 313 种至少含有两个不同肽段的蛋白质。鉴定出的蛋白质包括 89 种具有预测信号肽的蛋白质和 25 种预测定位于膜上的蛋白质。此外，有 67 种蛋白质具有碱性等电点值。我们使用谱图和肽段计数比较了两种不同条件下的蛋白质丰度：在丰富培养基中的生长和在小鼠消化道中的运输后。我们鉴定出大机械敏感通道和一种假定的阳离子转运体作为细菌适应消化道的膜标记物。
