Institute of Life and Health Engineering/National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, People's Republic of China.
OMICS. 2011 Jul-Aug;15(7-8):477-82. doi: 10.1089/omi.2010.0113. Epub 2011 Jun 23.
Streptococcus pneumoniae is a major human respiratory pathogen causing considerable morbidity and mortality worldwide. In order to better understand the pathogenesis of S. pneumoniae, we employed SDS-PAGE combined with LC-MS/MS analysis and in-solution digestion coupled with 2D-LC-MS/MS to obtain the whole-cell proteome of the bacterium. Among the identified 1,210 proteins, 345 proteins were annotated for cellular components, 613 for biological processes, and 421 for molecular functions. Important virulence-associated surface proteins such as Eno, ZmpB, and PrtA were identified. Classification analysis and protein-protein interaction map revealed that these identified proteins are involved in many biological processes including protein biosynthesis, protein folding and proteolysis, cell cycle, or regulation and carbohydrate metabolism. These data represent a comprehensive reference map of S. pneumoniae proteome, providing a useful source for further analysis of the virulence factors and the regulatory network involved in the pathogenesis of the bacterium.
肺炎链球菌是一种主要的人类呼吸道病原体，在全球范围内造成了相当大的发病率和死亡率。为了更好地了解肺炎链球菌的发病机制，我们采用 SDS-PAGE 结合 LC-MS/MS 分析和溶液消化结合 2D-LC-MS/MS 方法，获得了该细菌的全细胞蛋白质组。在鉴定的 1210 种蛋白质中，有 345 种蛋白质被注释为细胞成分，613 种为生物过程，421 种为分子功能。鉴定出了重要的与毒力相关的表面蛋白，如 Eno、ZmpB 和 PrtA。分类分析和蛋白质-蛋白质相互作用图谱表明，这些鉴定出的蛋白质参与了许多生物学过程，包括蛋白质生物合成、蛋白质折叠和蛋白水解、细胞周期或调节以及碳水化合物代谢。这些数据代表了肺炎链球菌蛋白质组的综合参考图谱，为进一步分析细菌发病机制中的毒力因子和调控网络提供了有用的资源。
