Roy Karine, Meyrand Mickaël, Corthier Gérard, Monnet Véronique, Mistou Michel-Yves
INRA, Unité de Biochimie Bactérienne, Centre de Recherches de Jouy-en-Josas, Jouy-en-Josas, France.
Proteomics. 2008 Apr;8(8):1661-76. doi: 10.1002/pmic.200700698.
Lactic acid bacteria are used on an industrial scale for the manufacturing of dairy products. It is now intended to develop novel applications of lactic acid bacteria that could be used as living vehicles for the targeting of antigens or therapeutics to the digestive mucosa. The aim of this study was to analyze the adaptations of Lactococcus lactis, a model lactic acid bacteria to the digestive tract and to identify functions required for colonization of the intestine. For this purpose, we combined gnotobiology with proteomics: axenic mice were colonized with a dairy L. lactis strain and the bacterial proteome was examined by 2-DE. As compared to cultures in broth, the proteome profile of bacteria grown in the intestine indicates the activation of metabolic pathways involved in various carbon sources assimilation and suggests the adoption of a mixed acids fermentative metabolism. We identified the product of the ywcC gene as essential for the colonization of the digestive tract and demonstrated that the corresponding gene product (YwcC) possesses a phosphogluconolactonase activity, suggesting an important role of the pentose phosphate pathway for the development of L. lactis in the digestive environment.
乳酸菌在工业规模上用于乳制品的生产。目前旨在开发乳酸菌的新应用，其可用作将抗原或治疗剂靶向消化粘膜的活载体。本研究的目的是分析乳酸乳球菌（一种典型的乳酸菌）对消化道的适应性，并确定肠道定植所需的功能。为此，我们将悉生生物学与蛋白质组学相结合：无菌小鼠用一株乳制品乳酸乳球菌菌株定植，并通过二维电泳检查细菌蛋白质组。与肉汤培养相比，在肠道中生长的细菌的蛋白质组谱表明参与各种碳源同化的代谢途径被激活，并表明采用了混合酸发酵代谢。我们确定ywcC基因的产物对消化道定植至关重要，并证明相应的基因产物（YwcC）具有磷酸葡萄糖酸内酯酶活性，这表明磷酸戊糖途径在乳酸乳球菌在消化环境中的发育中起重要作用。
