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[鸡胚细胞中劳斯病毒特异性RNA合成的研究]

[Study of the synthesis of Rous virus-specific RNA in chick embryo cells].

作者信息

Tatosian A G, Iakovleva L S, Semenova L A, Kiselev F L

出版信息

Vopr Virusol. 1977 Jul-Aug(4):460-4.

PMID:200014
Abstract

The presence of virus-specific RNA in commercial chick embryos and its lack in chick embryos of leukemia-free chicken farm of the USSR AMS Oncological Research Center as well as in cell cultures from RIF-free chicken infected with Marek's disease virus was demonstrated by hybridizationof 3H-DNA-product of Rous sarcoma virus synthesized in vitro in the presence of actinomycin D with the total preparation of cellular RNA.

摘要

通过在放线菌素D存在的情况下体外合成的劳斯肉瘤病毒的3H-DNA产物与细胞RNA的总制剂进行杂交,证明了商品鸡胚中存在病毒特异性RNA,而苏联医学科学院肿瘤研究中心无白血病鸡场的鸡胚以及感染马立克氏病病毒的无RIF鸡的细胞培养物中则不存在这种病毒特异性RNA。

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