Varmus H E, Weiss R A, Friis R R, Levinson W, Bishop J M
Proc Natl Acad Sci U S A. 1972 Jan;69(1):20-4. doi: 10.1073/pnas.69.1.20.
The effect of unlabeled cellular DNA upon the reassociation kinetics of labeled double-stranded DNA made by DNA polymerase from avian tumor viruses has been used to measure virus-specific nucleotide sequences in cells. Multiple copies of these sequences were found equally in normal chick cells, in chick cells transformed in culture, and in Rous tumor cells. Copies were also present equally in cells harboring chick helper factor and group-specific antigen, and in cells lacking those characteristics. Quail DNA also contains multiple copies of these sequences, but no copies were detected in HeLa-cell DNA or salmon-sperm DNA.
未标记的细胞DNA对禽肿瘤病毒DNA聚合酶合成的标记双链DNA重缔合动力学的影响已被用于测量细胞中的病毒特异性核苷酸序列。在正常鸡细胞、培养中转化的鸡细胞和劳氏肿瘤细胞中均发现了这些序列的多个拷贝。在含有鸡辅助因子和群特异性抗原的细胞以及缺乏这些特征的细胞中，拷贝数也相同。鹌鹑DNA也含有这些序列的多个拷贝，但在HeLa细胞DNA或鲑鱼精子DNA中未检测到拷贝。
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