Stem Cell Transplant Unit, Aghia Sophia Children's Hospital, Athens, Greece.
Clin Chem Lab Med. 2010 Feb;48(2):199-203. doi: 10.1515/CCLM.2010.034.
Exercise in humans augments the mobilization of circulating hematopoietic progenitor cells (CD34(+)) from the bone marrow. We investigated the effect of inflammation on erythroid marrow activity by mobilization of erythroid progenitor cells (EPs) along with soluble markers of erythropoiesis.
Ten healthy athletes who participated in an ultradistance foot race participated in the study. Peripheral blood mononuclear cells were isolated, before (phase I), at the end (phase II), and at 48 h post-race (phase III). EPs were detected as burst colony forming units (BFU-e) and colonies were scored at day 14. Markers of inflammation (C-reactive protein, serum amyloid-A, interleukin-6, ferritin and S100B) and bone marrow activity (erythropoietin, soluble transferrin receptor and lipocalin-2) were assessed.
An approximately three-fold decrease in BFU-e number was observed at phase II. sTfR concentrations were also decreased at phase II and remained decreased at phase III. However, EPO and lipocalin-2 concentrations reached a maximum value at phase II, with a tendency to decrease at phase III.
These findings indicate that exercise-induced inflammation modulates bone marrow homeostasis leading to an increase in leukocyte turnover and a decrease in erythroid compartment. It appears that lipocalin-2 is the main factor that regulates the production and mobilization of EPs.
人体运动可增强循环造血祖细胞（CD34(+））从骨髓中的动员。我们通过动员红系祖细胞（EPs）以及红系生成的可溶性标志物来研究炎症对红骨髓活性的影响。
10 名参加超长距离跑步比赛的健康运动员参加了这项研究。在运动前（第 I 期）、运动结束时（第 II 期）和运动后 48 小时（第 III 期）分离外周血单核细胞。检测爆式集落形成单位（BFU-e）作为 EP，并在第 14 天对集落进行评分。评估炎症标志物（C 反应蛋白、血清淀粉样蛋白 A、白细胞介素 6、铁蛋白和 S100B）和骨髓活性（促红细胞生成素、可溶性转铁蛋白受体和脂联素-2）。
在第 II 期，BFU-e 的数量减少了约三倍。sTfR 浓度在第 II 期也降低，并在第 III 期持续降低。然而，EPO 和脂联素-2 浓度在第 II 期达到最大值，在第 III 期有下降趋势。
这些发现表明，运动引起的炎症调节了骨髓的动态平衡，导致白细胞周转率增加和红系细胞减少。似乎脂联素-2是调节 EP 的产生和动员的主要因素。
