Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA.
J Vet Intern Med. 2010 Jan-Feb;24(1):127-31. doi: 10.1111/j.1939-1676.2009.0440.x.
A large unnamed Babesia species was detected in a dog with lymphoma. It was unknown if this was an underrecognized pathogen.
Report the historical and clinicopathologic findings in 7 dogs with babesiosis caused by a large unnamed Babesia species characterize the 18S ribosomal ribonucleic acid (rRNA) genes.
Seven immunocompromised dogs from which the Babesia was isolated.
Retrospective case review. Cases were identified by a diagnostic laboratory, the attending clinicians were contacted and the medical records were reviewed. The Babesia sp. 18S rRNA genes were amplified and sequenced.
Six of 7 dogs had been splenectomized; the remaining dog was receiving oncolytic drugs. Lethargy, anorexia, fever, and pigmenturia were reported in 6/7, 6/7, 4/7, and 3/7 dogs. Laboratory findings included mild anemia (7/7) and severe thrombocytopenia (6/7). Polymerase chain reaction (PCR) assays used to detect Babesia sensu stricto species were all positive, but specific PCR assays for Babesia canis and Babesia gibsoni were negative in all dogs. The 18S rRNA gene sequences were determined to be identical to a large unnamed Babesia sp. previously isolated. Cross-reactive antibodies against other Babesia spp. were not always detectable. Five dogs were treated with imidocarb dipropionate and 1 dog with atovaquone/azithromycin; some favorable responses were noted. The remaining dog was untreated and remained a clinically stable carrier.
Dogs with pigmenturia, anemia, and thrombocytopenia should be tested for Babesia sp. by PCR. Serology is not sufficient for diagnosis of this Babesia sp. Asplenia, chemotherapy, or both might represent risk factors for persistent infection, illness, or both.
在一只患有淋巴瘤的狗中检测到一种大型未命名的巴贝斯虫。尚不清楚这是否是一种未被识别的病原体。
报告 7 只由大型未命名巴贝斯虫引起的巴贝斯虫病的历史和临床病理发现，描述 18S 核糖体核糖核酸（rRNA）基因。
从 7 只免疫功能低下的狗中分离出巴贝斯虫。
回顾性病例回顾。通过诊断实验室确定病例，联系主治临床医生并审查病历。扩增和测序巴贝斯虫 sp.18S rRNA 基因。
7 只狗中有 6 只接受了脾切除术；其余一只正在接受肿瘤溶解药物治疗。6/7 只狗出现嗜睡、厌食、发热和色素尿，6/7 只狗出现轻度贫血，6/7 只狗出现严重血小板减少。聚合酶链反应（PCR）检测用于检测巴贝斯虫属严格意义上的物种均为阳性，但所有犬均为巴贝斯虫犬和巴贝斯虫吉氏的特异性 PCR 检测均为阴性。18S rRNA 基因序列与先前分离的大型未命名巴贝斯虫 sp. 完全相同。并非总是能检测到针对其他巴贝斯虫属的交叉反应性抗体。5 只狗用咪唑苯并二氮卓二丙酸酯治疗，1 只狗用阿托伐醌/阿奇霉素治疗；有些反应良好。剩下的狗未接受治疗，仍然是临床稳定的携带者。
出现色素尿、贫血和血小板减少的狗应通过 PCR 检测巴贝斯虫 sp。血清学不足以诊断这种巴贝斯虫属。脾切除术、化疗或两者兼而有之可能是持续感染、疾病或两者的危险因素。
