Centre de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
HIV Med. 2010 Apr;11(4):260-5. doi: 10.1111/j.1468-1293.2009.00772.x. Epub 2009 Dec 3.
HIV infection and its treatment are associated with dyslipidaemia and increased risk of cardiovascular disease. Accurate high-density lipoprotein (HDL) cholesterol values are necessary for the management of these abnormalities, but current methods have not been properly assessed in these patients. The aim of this study was to assess in HIV-infected patients the consistency and accuracy of a synthetic polymer/detergent homogeneous assay used to measure HDL cholesterol concentrations and to evaluate the impact of storage.
HDL cholesterol was measured using a synthetic polymer/detergent homogeneous method in samples from HIV-infected patients and healthy subjects for each of the storage regimens: baseline, after 1 week at 4 degrees C, and after 12 months at -80 degrees C. The ultracentrifugation and precipitation assays were used for comparison.
Three out of every 20 samples from HIV-infected patients had discrepant HDL cholesterol values with respect to the ultracentrifugation method. Overestimation was associated with high C-reactive protein concentrations and underestimation with plasma gamma-globulin concentrations, an effect that was amplified by any of the storage conditions tested.
Caution is needed when using the synthetic polymer/detergent homogeneous method for direct measurement of HDL cholesterol concentrations in HIV-infected patients. This assay is of limited use in clinical trials in which frozen samples are analysed.
HIV 感染及其治疗与血脂异常和心血管疾病风险增加有关。准确的高密度脂蛋白（HDL）胆固醇值对于这些异常的管理是必要的，但目前的方法尚未在这些患者中得到适当评估。本研究旨在评估在 HIV 感染患者中使用合成聚合物/洗涤剂均相测定法测量 HDL 胆固醇浓度的一致性和准确性，并评估储存的影响。
使用合成聚合物/洗涤剂均相法测量 HIV 感染患者和健康受试者的 HDL 胆固醇浓度，对于每种储存方案：基线、4°C 下 1 周后和-80°C 下 12 个月后。比较超速离心和沉淀法。
在 HIV 感染患者的 20 个样本中，有 3 个样本的 HDL 胆固醇值与超速离心法不一致。高 C 反应蛋白浓度与高估相关，血浆γ球蛋白浓度与低估相关，这种影响在任何一种测试的储存条件下都会放大。
在 HIV 感染患者中直接测量 HDL 胆固醇浓度时，使用合成聚合物/洗涤剂均相法需要谨慎。在冷冻样本进行分析的临床试验中，该测定法的应用受到限制。
