Jinam T A, Saitou N, Edo J, Mahmood A, Phipps M E
Division of Population Genetics, National Institute of Genetics, Mishima, Japan.
Tissue Antigens. 2010 Feb;75(2):151-8. doi: 10.1111/j.1399-0039.2009.01417.x. Epub 2009 Dec 14.
This is the first report of high-resolution human leukocyte antigen (HLA) typing in four indigenous groups in Malaysia. A total of 99 normal, healthy participants representing the Negrito (Jehai and Kensiu), Proto-Malay (Temuan) and a native group of Borneo (Bidayuh) were typed for HLA-A, -B, -DRB1 and -DQB1 genes using sequence-based typing. Eleven HLA-A, 26 HLA-B, 16 HLA-DRB1 and 14 HLA-DQB1 alleles were detected, including a new allele, HLA-B3589 in the Jehai. Highly frequent alleles were A2407, B1513, B1801, DRB10901, DRB11202, DRB11502, DQB10303 and DQB1*0502. Principal component analysis based on high-resolution HLA-A, -B and -DRB1 allele frequencies showed close affinities among all four groups, including the Negritos, with other Southeast Asian populations. These results showed the scope of HLA diversity in these indigenous minority groups and may prove beneficial for future disease association, anthropological and forensic studies.
这是关于马来西亚四个原住民群体高分辨率人类白细胞抗原（HLA）分型的首份报告。使用基于序列的分型方法，对总共99名代表尼格利陀人（杰海人和肯休人）、原马来人（特穆安人）以及婆罗洲一个原住民群体（比达友人）的正常健康参与者进行了HLA - A、- B、- DRB1和- DQB1基因分型。检测到11个HLA - A等位基因、26个HLA - B等位基因、16个HLA - DRB1等位基因和14个HLA - DQB1等位基因，其中包括杰海人中一个新的等位基因HLA - B3589。高频等位基因有A2407、B1513、B1801、DRB10901、DRB11202、DRB11502、DQB10303和DQB1*0502。基于高分辨率HLA - A、- B和- DRB1等位基因频率的主成分分析显示，所有四个群体，包括尼格利陀人，与其他东南亚人群之间具有密切的亲缘关系。这些结果显示了这些原住民少数群体中HLA多样性的范围，可能对未来的疾病关联、人类学和法医学研究有益。
