人羊膜干细胞在钛螺钉上的培养：骨工程的新视角。

Human amniotic fluid stem cells culture onto titanium screws: a new perspective for bone engineering.

出版信息

J Biol Regul Homeost Agents. 2009 Oct-Dec;23(4):277-9.

PMID:20003768

Abstract

The use of titanium plates and screws for osteosynthesis is considered to be an effective treatment for different kinds of fractures in orthopedic surgery. The aim of the present study is to test the ability of titanium screws to promote the growth of osteoblasts obtained from human amniotic fluid stem cells (AFS). Osteoblastic differentiation was assessed by RT-PCR of specific markers such as COL1, ONC, OPN, OCN, OPG, BMP-4 and Runx2. Mineralization was demonstrated by the presence of red depositions. Adherent cells were found to cover the whole surface of titanium screw by Scanning Electron Microscopy (SEM). The result indicates the excellent growth of osteoblasts obtained from amniotic fluid on a titanium surface and could represent an important point in view of a possible therapeutic application of AFS cells.

摘要

在骨科手术中，使用钛板和螺钉进行骨接合被认为是一种有效的治疗各种骨折的方法。本研究的目的是测试钛螺钉促进从人羊水干细胞（AFS）获得的成骨细胞生长的能力。通过 RT-PCR 检测 COL1、ONC、OPN、OCN、OPG、BMP-4 和 Runx2 等特异性标志物来评估成骨细胞分化。通过存在红色沉积物来证明矿化。扫描电子显微镜（SEM）观察到贴壁细胞覆盖在钛螺钉的整个表面。结果表明，从羊水获得的成骨细胞在钛表面上的生长良好，这可能是 AFS 细胞可能的治疗应用的一个重要方面。