Meirelles Flávio V, Birgel Eduardo H, Perecin Felipe, Bertolini Marcelo, Traldi Anneliese S, Pimentel José Rodrigo V, Komninou Eliza R, Sangalli Juliano R, Neto Paulo Fantinato, Nunes Mariana Tikuma, Pogliani Fábio Celidonio, Meirelles Flávia D P, Kubrusly Flávia S, Vannucchi Camila I, Silva Liege C G
Faculty of Animal Sciences and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225. Pirassununga, SP 13635-900, Brazil.
Reprod Fertil Dev. 2010;22(1):88-97. doi: 10.1071/RD09229.
The production of a healthy cloned calf is dependent on a multitude of successful steps, including reprogramming mediated by the oocyte, the development of a functional placenta, adequate maternal-fetal interaction, the establishment of a physiological metabolic setting and the formation of a complete set of well-differentiated cells that will eventually result in well-characterised and fully competent tissues and organs. Although the efficiency of nuclear transfer has improved significantly since the first report of a somatic cell nuclear transfer-derived animal, there are many descriptions of anomalies concerning cloned calves leading to high perinatal morbidity and mortality. The present article discusses some our experience regarding perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has led to improved survival rates in Nellore cloned calves following the application of such 'labour-intensive technology'.
健康克隆牛犊的产生依赖于众多成功步骤，包括由卵母细胞介导的重编程、功能性胎盘的发育、充足的母胎相互作用、生理代谢环境的建立以及一整套充分分化细胞的形成，这些细胞最终将形成特征明确且功能完备的组织和器官。尽管自从首次报道体细胞克隆动物以来，核移植效率有了显著提高，但仍有许多关于克隆牛犊异常情况的描述，这些异常导致围产期发病率和死亡率居高不下。本文讨论了我们在克隆瘤牛（印度瘤牛）围产期和新生儿期操作方面的一些经验，应用这种“劳动密集型技术”后，内洛尔克隆牛犊的存活率得到了提高。
