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蛋白激酶C抑制剂可阻止海兔B型光感受器中细胞记忆的诱导和持续表达。

Protein kinase C inhibitors prevent induction and continued expression of cell memory in Hermissenda type B photoreceptors.

作者信息

Farley J, Schuman E

机构信息

Program in Neural Science, Indiana University, Bloomington 47405.

出版信息

Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):2016-20. doi: 10.1073/pnas.88.5.2016.

DOI:10.1073/pnas.88.5.2016
PMID:2000409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC51157/
Abstract

Injections of cAMP-dependent, Ca2+/calmodulin-dependent, or Ca2+/phospholipid-dependent protein kinases into Hermissenda crassicornis type B photoreceptors are sufficient to induce many of the changes in B-cell excitability produced by associative conditioning. We report that inhibitors of Ca2+/phospholipid-dependent protein kinases, but not inhibitors of cyclic nucleotide- or Ca2+/calmodulin-dependent protein kinases, prevent the induction as well as continued expression of learning-produced changes in type-B-cell excitability: reductions of voltage-dependent and Ca2(+)-activated K+ currents. Our results represent a direct demonstration of long-term (days) experientially induced modulation of ion-channel activity that is dependent upon persistent kinase activity.

摘要

向肥颈艾氏海蛞蝓B型光感受器中注射环磷酸腺苷（cAMP）依赖性、Ca2+/钙调蛋白依赖性或Ca2+/磷脂依赖性蛋白激酶，足以诱导由联合条件作用产生的B细胞兴奋性的许多变化。我们报告称，Ca2+/磷脂依赖性蛋白激酶的抑制剂可阻止B型细胞兴奋性学习产生变化的诱导及持续表达，即电压依赖性和Ca2+激活的钾电流的减少，但环核苷酸或Ca2+/钙调蛋白依赖性蛋白激酶的抑制剂则无此作用。我们的结果直接证明了长期（数天）由经验诱导的离子通道活性调节依赖于持续的激酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/f8a4393a052e/pnas01055-0437-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/e43627f64526/pnas01055-0435-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/a32cec342e52/pnas01055-0435-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/1794f1b45ee7/pnas01055-0435-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/f8a4393a052e/pnas01055-0437-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/e43627f64526/pnas01055-0435-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/a32cec342e52/pnas01055-0435-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/1794f1b45ee7/pnas01055-0435-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c33/51157/f8a4393a052e/pnas01055-0437-a.jpg

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Protein synthesis required for long-term memory is induced by PKC activation on days before associative learning.长期记忆所需的蛋白质合成是在联想学习前几天由蛋白激酶C激活诱导产生的。
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