Wu Colleen, Alman Benjamin A
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, University of Toronto, Toronto Medical Discovery Tower, East Tower, 101 College Street, Toronto, Ont., Canada M5G 1L7.
Cancer Lett. 2008 Sep 8;268(1):1-9. doi: 10.1016/j.canlet.2008.03.048. Epub 2008 May 19.
Cancer stem cells (CSCs) are found in multiple tumor types. While the presence of surface markers selectively expressed on CSCs are used to isolate these cells, no marker or pattern of makers are known to prospectively identify CSCs in many tumor types. In such cases exploitation of stem cell characteristics can be used to identify CSCs and one such characteristic is the capacity to extrude dyes such as Hoechst 33342. Cell that exclude this dye are referred to as side population (SP) cells. These cells share characteristics of CSCs, specifically, they are enriched for tumor initiating capacity, they express stem-like genes, and they are resistant to chemotherapeutic drugs. Dye exclusion is a valuable technique as it identifies a unique population of cells with stem-like characteristics.
癌症干细胞（CSCs）存在于多种肿瘤类型中。虽然利用在CSCs上选择性表达的表面标志物来分离这些细胞，但在许多肿瘤类型中，尚无已知的标志物或标志物模式可用于前瞻性地识别CSCs。在这种情况下，利用干细胞特性可用于识别CSCs，其中一个特性是挤出染料（如Hoechst 33342）的能力。排除这种染料的细胞被称为侧群（SP）细胞。这些细胞具有CSCs的特征，具体而言，它们富含肿瘤起始能力，表达干细胞样基因，并且对化疗药物具有抗性。染料排除是一项有价值的技术，因为它可识别具有干细胞样特征的独特细胞群体。
