Institute for Clinical & Experimental Surgery, University of Saarland, 66421 Homburg/Saar, Germany.
Acta Biomater. 2010 Jun;6(6):2020-7. doi: 10.1016/j.actbio.2009.12.004. Epub 2009 Dec 21.
Scaffolds for bone tissue engineering should provide an osteoconductive surface to promote the ingrowth of new bone after implantation into bone defects. This may be achieved by hydroxyapatite loading of distinct scaffold biomaterials. Herein, we analyzed the in vitro and in vivo properties of a novel nanosize hydroxyapatite particles/poly(ester-urethane) (nHA/PU) composite scaffold which was prepared by a salt leaching-phase inverse process. Microtomography, scanning electron microscopy and X-ray spectroscopy analyses demonstrated the capability of the material processing to create a three-dimensional porous PU scaffold with nHA on the surface. Compared to nHA-free PU scaffolds (control), this modified scaffold type induced a significant increase in in vitro adsorption of model proteins. In vivo analysis of the inflammatory and angiogenic host tissue response to implanted nHA/PU scaffolds in the dorsal skinfold chamber model indicated that the incorporation of nHA particles into the scaffold material did not affect biocompatibility and vascularization when compared to control scaffolds. Thus, nHA/PU composite scaffolds represent a promising new type of scaffold for bone tissue engineering, combining the flexible material properties of PU with the advantage of an osteoconductive surface.
用于骨组织工程的支架应为植入骨缺损后促进新骨向内生长提供一个骨传导表面。这可以通过将特定支架生物材料负载羟磷灰石来实现。在此，我们分析了一种新型纳米级羟磷灰石颗粒/聚酯-氨基甲酸酯（nHA/PU）复合支架的体外和体内特性，该支架是通过盐浸出-相反转工艺制备的。微断层扫描、扫描电子显微镜和 X 射线能谱分析表明，该材料处理方法能够在三维多孔 PU 支架表面形成 nHA。与不含 nHA 的 PU 支架（对照）相比，这种改良的支架类型显著增加了模型蛋白的体外吸附。在背部皮褶室模型中植入 nHA/PU 支架后对宿主组织炎症和血管生成反应的体内分析表明，与对照支架相比，将 nHA 颗粒掺入支架材料中并不影响生物相容性和血管化。因此，nHA/PU 复合支架代表了一种有前途的新型骨组织工程支架，将 PU 的柔性材料特性与骨传导表面的优势相结合。
