在 BCG 中表达的具有相同特征的细胞内或分泌蛋白中的 T 细胞表位 SIINFEKL 可诱导不同的 CD8+ T 细胞反应。
The same well-characterized T cell epitope SIINFEKL expressed in the context of a cytoplasmic or secreted protein in BCG induces different CD8+ T cell responses.
机构信息
Department of Pathology and Laboratory Sciences, University of Wisconsin, Madison, WI 53706, USA.
出版信息
Immunol Lett. 2010 May 4;130(1-2):36-42. doi: 10.1016/j.imlet.2009.12.004. Epub 2009 Dec 11.
Abstract
Mycobacterium bovis BCG is still the most widely used vaccine against tuberculosis and CD8(+) T cells play important roles in fighting infection. We investigated how well antigen is processed and presented to CD8(+) T cells using the same well-characterized CD8(+) T cell epitope SIINFEKL expressed in either a cytoplasmic (GFP-OVA) or secreted (85B-OVA) context from BCG. We report that secreted SIINFEKL from 85B-OVA BCG is presented better than cytoplasmic SIINFEKL expressed by GFP-OVA BCG.
摘要
牛型分枝杆菌卡介苗仍然是最广泛使用的结核病疫苗，CD8(+) T 细胞在抗感染中发挥重要作用。我们研究了用在牛型分枝杆菌中表达的同一经过充分鉴定的 CD8(+) T 细胞表位 SIINFEKL（以胞质（GFP-OVA）或分泌（85B-OVA）形式）来处理和呈递抗原的效果如何。我们报告称，85B-OVA 牛型分枝杆菌分泌的 SIINFEKL 比 GFP-OVA 牛型分枝杆菌表达的胞质 SIINFEKL 呈递得更好。
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