Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Anal Chim Acta. 2010 Jan 11;657(2):147-53. doi: 10.1016/j.aca.2009.10.035.
Voltammetric investigation of two corticoid isomers--testosterone and epitestosterone has been carried out at bare and single-wall carbon nanotubes (SWNT)-modified edge plane pyrolytic graphite electrode (EPPGE). Square wave voltammetry (OSWV) has been used for the simultaneous determination of isomeric steroids. The reduction of the two isomers occurred in a pH dependent, 2e, 2H+ process and well-defined voltammetric peaks were observed. Under the optimum experimental conditions, linear calibration curves are obtained within the concentration range 5-1000 nM for both the steroids with the limit of detection 2.8 x 10(-9) and 4.1 x 10(-9) M for testosterone and epitestosterone respectively. The developed protocol is successfully implemented for the analysis of both the compounds in the urine samples of normal subjects as well as in patients undergoing treatment with testosterone. The results obtained from the proposed voltammetric method were also compared with HPLC analysis and found to be similar.
已在裸电极和单壁碳纳米管（SWNT）修饰的边缘平面石墨电极（EPPGE）上对两种皮质甾族异构体——睾丸激素和表睾甾酮进行了伏安法研究。方波伏安法（OSWV）用于同时测定异构体类固醇。两种异构体的还原在 pH 依赖性、2e、2H+过程中发生，并且观察到了明确的伏安峰。在最佳实验条件下，两种类固醇在 5-1000 nM 浓度范围内均获得线性校准曲线，睾丸激素和表睾甾酮的检测限分别为 2.8×10(-9)和 4.1×10(-9) M。所开发的方案成功地用于分析正常受试者尿液样本中的这两种化合物，以及正在接受睾丸激素治疗的患者的尿液样本中的这两种化合物。从所提出的伏安法获得的结果也与 HPLC 分析进行了比较，结果相似。
