Center of Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou 510632, PR China.
Anal Chim Acta. 2010 Jan 11;657(2):210-4. doi: 10.1016/j.aca.2009.10.045.
In the current study, we developed a nanosphere bio-barcode technology to detect trace gonyautoxin 2/3 (GTX 2/3). GTX 2/3-glucose oxidase (GOX) conjugates were first prepared as the coating antigen in a periodate reaction. Subsequently, gold nanoparticles (NP) dual-labeled with anti-GTX 2/3 monoclonal antibodies (Mab) and DNA oligonucleotides were synthesized via a one-step preparation method. Combining PCR with indirect competitive ELISA (icELISA), a novel immunosorbent bio-barcode assay was established utilizing the Mab-NP-dsDNA complex to convert enzymatic signals to DNA signals. Importantly, the limit of detection of the method was lower than 0.74 microg mL(-1). Thus, the immunosorbent bio-barcode assay is a rapid and high-throughput screening tool to detect GTX 2/3 in aquatic products.
在本研究中，我们开发了一种纳米球生物条码技术来检测痕量冈田酸 2/3（GTX 2/3）。GTX 2/3-葡萄糖氧化酶（GOX）缀合物首先通过过碘酸盐反应制备为包被抗原。随后，通过一步制备方法合成了双标记有抗 GTX 2/3 单克隆抗体（Mab）和 DNA 寡核苷酸的金纳米粒子（NP）。通过将 PCR 与间接竞争 ELISA（icELISA）相结合，利用 Mab-NP-dsDNA 复合物将酶信号转换为 DNA 信号，建立了一种新型免疫吸附生物条码测定法。重要的是，该方法的检测限低于 0.74 μg mL（-1）。因此，免疫吸附生物条码测定法是一种快速高通量筛选工具，可用于检测水产品中的 GTX 2/3。
