Wang Yuanshang, Jin Maojun, Chen Ge, Cui Xueyan, Zhang Yudan, Li Mingjie, Liao Yun, Zhang Xiuyuan, Qin Guoxin, Yan Feiyan, Abd El-Aty A M, Wang Jing
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China.
Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, PR China.
J Adv Res. 2019 Apr 27;20:23-32. doi: 10.1016/j.jare.2019.04.009. eCollection 2019 Nov.
With the rapid development of nanotechnology, the bio-barcode assay (BCA), as a new diagnostic tool, has been gradually applied to the detection of protein and nucleic acid targets and small-molecule compounds. BCA has the advantages of high sensitivity, short detection time, simple operation, low cost, good repeatability and good linear relationship between detection results. However, bio-barcode technology is not yet fully formed as a complete detection system, and the detection process in all aspects and stages is unstable. Therefore, studying the optimal reaction conditions, optimizing the experimental steps, exploring the multi-residue detection of small-molecule substances, and preparing immuno-bio-barcode kits are important research directions for the standardization and commercialization of BCA. The main theme of this review was to describe the principle of BCA, provide a comparison of its application, and introduce the single-residue and multi-residue detection of macromolecules and single-residue detection of small molecules. We also compared it with other detection methods, summarized its feasibility and limitations, expecting that with further improvement and development, the technique can be more widely used in the field of stable small-molecule and multi-residue detection.
随着纳米技术的迅速发展，生物条形码分析（BCA）作为一种新型诊断工具，已逐渐应用于蛋白质、核酸靶标及小分子化合物的检测。BCA具有灵敏度高、检测时间短、操作简单、成本低、重复性好以及检测结果线性关系良好等优点。然而，生物条形码技术作为一个完整的检测系统尚未完全成型，各个方面和阶段的检测过程都不稳定。因此，研究最佳反应条件、优化实验步骤、探索小分子物质的多残留检测以及制备免疫生物条形码试剂盒是BCA标准化和商业化的重要研究方向。本综述的主题是描述BCA的原理，比较其应用情况，并介绍大分子的单残留和多残留检测以及小分子的单残留检测。我们还将其与其他检测方法进行了比较，总结了其可行性和局限性，期望随着进一步的改进和发展，该技术能够在稳定的小分子和多残留检测领域得到更广泛的应用。
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