间皮瘤中未检测到 Merkel 细胞多瘤病毒。
Merkel cell polyomavirus is not detected in mesotheliomas.
机构信息
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD, USA.
出版信息
J Clin Virol. 2010 Feb;47(2):196-8. doi: 10.1016/j.jcv.2009.11.019. Epub 2009 Dec 16.
BACKGROUND
Merkel cell polyomavirus (MCPyV) is the first polyoma virus consistently linked to the etiology of a human cancer. Serological studies indicate that the virus is commonly acquired in childhood, with seroprevalence reaching 50% or higher among young adults. The modes of MCPyV transmission are still unclear, but it has been identified in respiratory tract samples. Given its respiratory tropism, we examined whether MCPyV could be detected in mesothelioma tissue, a malignancy induced in animal models by another polyomavirus, SV40.
OBJECTIVE
To determine if MCPyV DNA can be detected in mesothelioma.
STUDY DESIGN
DNA was extracted from 45 fresh-frozen mesothelioma samples. PCR was used to detect and quantify the abundance of MCPyV DNA, and a human control gene, in duplicates of the tissues. DNA from a sequence verified MCC tumor was used as a positive control.
RESULTS
The human control gene was detected at high levels in all but three mesothelioma tissues. MCPyV DNA was detected in only one mesothelioma, and the level of viral DNA was very low.
CONCLUSIONS
These results are inconsistent with the hypothesis that MCPyV is etiologically linked to mesothelioma.
背景
默克尔细胞多瘤病毒（MCPyV）是首个与人类癌症病因明确相关的多瘤病毒。血清学研究表明，该病毒在儿童时期普遍感染，在年轻人中的血清阳性率达到 50%或更高。MCPyV 的传播模式仍不清楚，但已在呼吸道样本中被发现。鉴于其呼吸道趋向性，我们研究了默克尔细胞多瘤病毒是否可以在间皮瘤组织中检测到，间皮瘤是另一种多瘤病毒 SV40 在动物模型中诱导的恶性肿瘤。
目的
确定 MCPyV DNA 是否可以在间皮瘤中检测到。
研究设计
从 45 个新鲜冷冻的间皮瘤样本中提取 DNA。PCR 用于检测和定量组织中 MCPyV DNA 和人类对照基因的丰度。经序列验证的 MCC 肿瘤 DNA 用作阳性对照。
结果
除了三个间皮瘤组织外，所有组织中都检测到高水平的人类对照基因。仅在一个间皮瘤中检测到 MCPyV DNA，病毒 DNA 水平非常低。
结论
这些结果与默克尔细胞多瘤病毒与间皮瘤病因学相关的假设不一致。
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