前交叉韧带来源的细胞具有较高的软骨生成潜力。

Anterior cruciate ligament-derived cells have high chondrogenic potential.

机构信息

Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikatacho, Kitaku, Okayama 700-8558, Japan.

出版信息

Biochem Biophys Res Commun. 2010 Jan 1;391(1):1142-7. doi: 10.1016/j.bbrc.2009.12.044. Epub 2009 Dec 16.

DOI:10.1016/j.bbrc.2009.12.044

PMID:20006576

Abstract

Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and alpha1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.

摘要

前交叉韧带（ACL）来源的细胞具有不同于内侧副韧带（MCL）来源的细胞的特征。然而，在 ACL 和 MCL 的愈合潜力和细胞反应方面，其关键区别仍不清楚。本研究旨在研究每个韧带来源细胞的间充质分化特性。两种韧带来源的细胞均分化为成脂、成骨和成软骨谱系。在软骨形成中，ACL 来源的细胞比 MCL 来源的细胞具有更高的软骨生成特性。软骨形成标志物 Sox9 和 alpha1(II)胶原（Col2a1）在 ACL 来源的微球中比在 MCL 来源的微球中更快地诱导。Sox9 的表达先于 Col2a1 在两种微球培养细胞中的增加。然而，Sox9 和韧带/肌腱转录因子 Scleraxis 的表达水平并没有随着软骨形成诱导而与 Col2a1 表达的增加平行。本研究表明，Sox9 和 Scleraxis 之间的平衡在韧带来源细胞的软骨分化中起着重要作用。