School of Life and Environmental Sciences, Deakin University, Burwood, Victoria, Australia.
FEBS Lett. 2010 Feb 5;584(3):612-8. doi: 10.1016/j.febslet.2009.12.013. Epub 2009 Dec 17.
Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.
二十二碳六烯酸（DHA）是神经元细胞膜中主要的多不饱和脂肪酸。我们假设 DHA 通过降低 ZnT3 表达和锌摄取来减少神经元细胞死亡。与 DHA 充足细胞相比，将 M17 细胞暴露于 DHA 缺乏培养基中会增加活性 caspase-3 的水平，证实 DHA 缺乏对促进神经元细胞死亡的不利影响。与 DHA 耗尽细胞相比，在 DHA 处理的 M17 细胞中，锌摄取减少了 65%，并且 ZnT3 mRNA 和蛋白水平降低。我们提出，DHA 的神经保护功能是通过降低细胞内锌水平来发挥的，从而抑制细胞凋亡。
