MacPhee Daniel J
Division of BioMedical Sciences, Faculty of Medicine, Health Sciences Centre, Memorial University of Newfoundland, St. John's, NL, Canada A1B 3V6.
J Pharmacol Toxicol Methods. 2010 Mar-Apr;61(2):171-7. doi: 10.1016/j.vascn.2009.12.001. Epub 2009 Dec 23.
The need for a technique that could allow the determination of antigen specificity of antisera led to the development of a method that allowed the production of a replica of proteins, which had been separated electrophoretically on polyacrylamide gels, on to a nitrocellulose membrane. This method was coined Western blotting and is very useful to study the presence, relative abundance, relative molecular mass, post-translational modification, and interaction of specific proteins. As a result it is utilized routinely in many fields of scientific research such as chemistry, biology and biomedical sciences. This review serves to touch on some of the methodological conditions that should be considered to improve Western blot analysis, particularly as a guide for graduate students but also scientists who wish to continue adapting this now fundamental research tool.
对一种能够确定抗血清抗原特异性的技术的需求，促使人们开发出一种方法，该方法可将在聚丙烯酰胺凝胶上通过电泳分离的蛋白质复制品转移到硝酸纤维素膜上。这种方法被称为蛋白质免疫印迹法，对于研究特定蛋白质的存在、相对丰度、相对分子质量、翻译后修饰及相互作用非常有用。因此，它在化学、生物学和生物医学科学等许多科研领域中被常规使用。本综述旨在探讨一些在改进蛋白质免疫印迹分析时应考虑的方法学条件，特别是作为研究生以及希望继续应用这一基础研究工具的科学家的指南。
