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用于骨关节炎建模的复杂人类骨软骨外植体的采集、建立与评估

Collection, Establishment and Assessment of Complex Human Osteocartilaginous Explants for Modeling Osteoarthritis.

作者信息

Danceanu-Zara Camelia-Mihaela, Petrovici Adriana, Labusca Luminita, Minuti Anca Emanuela, Stavila Cristina, Plamadeala Petru, Tiron Crina Elena, Aniţă Dragoş, Aniţă Adriana, Lupu Nicoleta

机构信息

National Institute of Research and Development in Technical Physics, 700050 Iasi, Romania.

Transcend Center Regional Oncology Institute, 700483 Iasi, Romania.

出版信息

Biomedicines. 2024 Oct 21;12(10):2406. doi: 10.3390/biomedicines12102406.

DOI:10.3390/biomedicines12102406
PMID:39457719
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11504061/
Abstract

With the increasing burden of osteoarthritis worldwide, cost efficient and reliable models are needed to enable the development of innovative therapies or therapeutic interventions. Ex vivo models have been identified as valuable modalities in translational research, bridging the gap between in vitro and in vivo models. Osteocartilaginous explants from Osteoarthritis (OA) patients offer an exquisite opportunity for studying OA progression and testing novel therapies. We describe the protocol for establishing human osteocartilaginous explants with or without co-culture of homologous synovial tissue. Furthermore, a detailed protocol for the assessment of explanted tissue in terms of protein content using Western blot and immunohistochemistry is provided. Commentaries regarding the technique of choice, possible variations and expected results are inserted.

摘要

随着全球骨关节炎负担的日益加重，需要经济高效且可靠的模型来推动创新疗法或治疗干预措施的开发。体外模型已被视为转化研究中的重要手段，填补了体外和体内模型之间的空白。骨关节炎（OA）患者的骨软骨外植体为研究OA进展和测试新疗法提供了绝佳机会。我们描述了建立有或没有同源滑膜组织共培养的人骨软骨外植体的方案。此外，还提供了一份详细的方案，用于通过蛋白质免疫印迹和免疫组织化学评估外植组织的蛋白质含量。文中插入了关于所选技术、可能的变体和预期结果的评论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c6/11504061/483dbdd819da/biomedicines-12-02406-g007.jpg
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本文引用的文献

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Osteochondral Tissue-On-a-Chip: A Novel Model for Osteoarthritis Research.软骨组织芯片：用于骨关节炎研究的新型模型。
Int J Mol Sci. 2024 Sep 11;25(18):9834. doi: 10.3390/ijms25189834.
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Obesity, Metabolic Syndrome, and Osteoarthritis Require Integrative Understanding and Management.肥胖、代谢综合征与骨关节炎需要综合理解与管理。
Biomedicines. 2024 Jun 6;12(6):1262. doi: 10.3390/biomedicines12061262.
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Optimizing reduction of western blotting analytical variations: Use of replicate test samples, multiple normalization methods, and sample loading positions.
优化 Western blot 分析变异的减少：使用重复测试样本、多种归一化方法和样品加载位置。
Anal Biochem. 2023 Aug 1;674:115198. doi: 10.1016/j.ab.2023.115198. Epub 2023 Jun 10.
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Sirt6 attenuates chondrocyte senescence and osteoarthritis progression.Sirt6 可减轻软骨细胞衰老和骨关节炎进展。
Nat Commun. 2022 Dec 10;13(1):7658. doi: 10.1038/s41467-022-35424-w.
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Western blotting: a powerful staple in scientific and biomedical research.蛋白质免疫印迹法：科学和生物医学研究中一项强大的常用技术。
Biotechniques. 2022 Jun;73(1):58-69. doi: 10.2144/btn-2022-0003. Epub 2022 Jul 1.
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Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics.人骨软骨外植体作为骨关节炎的体外模型用于评估新型骨科生物制剂
Pharmaceutics. 2022 Jun 10;14(6):1231. doi: 10.3390/pharmaceutics14061231.
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Trends in Complications and Outcomes in Patients Aged 65 Years and Younger Undergoing Total Knee Arthroplasty: Data From the American Joint Replacement Registry.65 岁以下全膝关节置换术患者并发症和结局的趋势：来自美国关节置换登记处的数据。
J Am Acad Orthop Surg Glob Res Rev. 2022 Jun 15;6(6). doi: 10.5435/JAAOSGlobal-D-22-00116. eCollection 2022 Jun 1.
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AAOS Clinical Practice Guideline Summary: Management of Osteoarthritis of the Knee (Nonarthroplasty), Third Edition.AAOS 临床实践指南摘要：膝关节骨关节炎（非关节置换）的治疗，第三版。
J Am Acad Orthop Surg. 2022 May 1;30(9):e721-e729. doi: 10.5435/JAAOS-D-21-01233.
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Global, regional, and national burden of diseases and injuries for adults 70 years and older: systematic analysis for the Global Burden of Disease 2019 Study.全球、区域和国家 70 岁及以上成年人的疾病和伤害负担：全球疾病负担 2019 研究的系统分析。
BMJ. 2022 Mar 10;376:e068208. doi: 10.1136/bmj-2021-068208.
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