Pal Jayanta K, Berwal Sunil K, Soni Rupali N
University of Pune, Pune, India.
Methods Mol Biol. 2012;869:551-9. doi: 10.1007/978-1-61779-821-4_49.
A simple method for staining of proteins simultaneously on sodium dodecyl sulfate (SDS) polyacrylamide gels and nitrocellulose membranes by Alta during western blot analysis is described. A 5% solution of Alta, a commercially available cosmetic preparation, is added in the upper tank buffer during electrophoresis. On completion of electrophoresis, the gel is washed in distilled water and viewed on a white light plate and a transilluminator to photograph the protein profiles. The gel is processed for western blot transfer of proteins onto a nitrocellulose membrane, and upon completion, the protein profiles on the membrane are viewed and photographed as stated above. The membrane can then be processed for immunostaining as per the standard procedure. Thus, the staining procedure using Alta is simple, rapid (without any need of destaining), and cost-effective.
本文描述了一种在蛋白质印迹分析过程中，通过阿尔塔（Alta）同时对十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶和硝酸纤维素膜上的蛋白质进行染色的简单方法。一种市售的化妆品制剂阿尔塔的5%溶液在电泳过程中添加到上槽缓冲液中。电泳完成后，凝胶在蒸馏水中洗涤，并在白光板和透射仪上观察，以拍摄蛋白质条带图。将凝胶进行处理，以便将蛋白质印迹转移到硝酸纤维素膜上，完成后，如上述那样观察并拍摄膜上的蛋白质条带图。然后可按照标准程序对膜进行免疫染色。因此，使用阿尔塔的染色程序简单、快速（无需脱色）且具有成本效益。
