Faculty of Sciences, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands.
Biophys J. 2009 Dec 16;97(12):3215-23. doi: 10.1016/j.bpj.2009.09.037.
Light harvesting complex II (LHCII) is the most abundant protein in the thylakoid membrane of higher plants and green algae. LHCII acts to collect solar radiation, transferring this energy mainly toward photosystem II, with a smaller amount going to photosystem I; it is then converted into a chemical, storable form. We performed time-resolved femtosecond visible pump/mid-infrared probe and visible pump/visible probe absorption difference spectroscopy on purified LHCII to gain insight into the energy transfer in this complex occurring in the femto-picosecond time regime. We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604.
捕光复合物 II（LHCII）是高等植物和绿藻类囊体膜中含量最丰富的蛋白质。LHCII 的作用是收集太阳辐射，将能量主要传递给光系统 II，一小部分传递给光系统 I；然后将其转化为可储存的化学形式。我们对纯化的 LHCII 进行了飞秒可见泵/中红外探测和可见泵/可见探测吸收差光谱实验，以深入了解在飞秒皮秒时间范围内该复合物中的能量转移。我们发现，从中红外光谱中获得的信息，以及结构和建模信息，为通过瓶颈色素叶绿素 a604 传递能量的情况提供了独特的可视化。
